Figure 2: Upregulated proteins in DLD-1 cells after siRNA knockdown of PSF. DLD-1 cells were transiently transfected with control siRNA or siRNA targeting PSF. MS/MS analyses were performed using the AB SCIEX TOF/TOF™ 5800 System (AB SCIEX, Foster City, CA, USA). Protein identification was performed through ProteinPilot™ software (AB Sciex, Framingham, MA, USA) using the UniProt database. We identified 25 distinct proteins whose levels were significantly altered following PSF knockdown in DLD-1 cells [34]. On the basis of these results, we suggest that PSF activity may influence protein translation, the cell cycle, apoptosis, and cytoskeleton dynamics.