Figure 2: Confirmation of stable BjAnn1 expression in human GBM cell lines. (A) Whole cell lysates from EV- and BjAnn1-expressing cells were subjected to Western blotting. Blots were stripped and re-probed with GAPDH to monitor equal loading. (B) Semi-quantitative RT-PCR was performed in EV- and BjAnn1- expressing U87 and U251 cells. (C) Confocal microscopy was performed as described in Materials and Methods. Representative micrographs of BjAnn1 (red) expression were presented from randomly selected microscopic fields. Nuclei were counterstained with DAPI.