Figure 3: Ectopic expression of BjAnn1 reduces the proliferation, migration and colony formation of GBM cell lines. (A) U87 and U251 cells were transfected with control (pEV) and BjAnn1 plasmids and the proliferation was determined at 72 h by using the MTT assay. All data presented are the mean of 3 independent experiments with mean ± SE. *, p < 0.05. (B) U87 and U251 cells stably expressing control (pEV) or BjAnn1 plasmids were seeded in 6 well plates (500 cells/well) and allowed to grow for 8 days. Colonies were stained with crystal violet and colonies that contain ≥50 cells were counted. All data presented are the mean of 3 independent experiments with mean ± SE. *, p < 0.05. (C) U87 and U251 GBM cells were transfected with control (pEV) and BjAnn1 plasmids were subjected scratch wound healing assay as described in materials and methods and the distance of migration was determined after 12 hours. (D) U87 and U251 cells transfected with control (pEV) and BjAnn1 plasmids were subjected to matrigel invasion assay and the percentage of invaded cells compared to control were plotted on histogram.