Gene NCBI REFSEQ Description Fold change: BjANN1/EV
AOX1 CCS CYB DGKK DUOX2 DUSP1 FOXM GPR156 GPX1 GPX2 GPX3 GSR GSS GSTZ1 MBL2 MPO MPV17 MSRA MT3 MTL5 NME5 PKP PRDX2 PRDX3 PREX1 PR3 PXDN SELS SIRT2 SOD2 SOD3 NP_001150.3 NP_005116.1 NP_536855.1 NP_001013764.1 NP_054799.4 NP_004408.1 NP_001230017.1 NP_001161743.1 NP_000572.2 NP_002074.2 NP_002075.2 NP_000628.2 NP_000169.1 NP_001504.2 NP_000233.1 NP_000241.1 NP_002428.1 NP_001129142.1 NP_005945.1 NP_004914.2 NP_003542.1 NP_000290.2 NP_005800.3 NP_006784.1 NP_065871.2 NP_002768.3 NP_036425.1 NP_060915.2 NP_001180215.1 NP_000627.2 NP_003093.2 Aldehyde oxidase 1 Superoxide dismutase copper chaperone Cytochrome b Diacylglycerol kinase, kappa NADPH oxidase Dual specificity phosphatase 1 Forkhead box M1 G protein-coupled receptor 15 Glutathione peroxidase 1 Glutathione peroxidase 2 Glutathione peroxidase 3 Glutathione reductase Glutathione synthetase Glutathione S-transferase zeta 1 Mannose-binding lectin (protein C) 2, soluble Myeloperoxidase MpV17 mitochondrial inner membrane protein Methionine sulfoxide reductase A Metallothionein 3 Metallothionein-like 5, testis-specific (tesmin) NME/NM23 family member Plakophilin 1 Peroxiredoxin 2 Peroxiredoxin 3 PtdIns(3,4,5)-dependent Rac exchanger 1 Proteinase 3 Peroxidasin homolog Selenoprotein S Sirtuin 2 Mn superoxide dismutase Extracellular superoxide dismutase [Cu-Zn] 3.13 5.03 4.25 3.51 4.28 3.23 4.04 3.43 3.22 3.97 3.26 3.96 4.95 3.05 4.20 3.16 3.55 3.75 5.43 6.34 7.74 3.59 3.44 3.03 3.14 3.23 5.33 7.04 3.72 6.42 3.94
Table 1: Modulation of oxidative stress response genes in BjANN1-expressing cells. Total RNA was isolated from EV- and BjANN1-stable expressing cells and cDNAs were subjected to Human Oxidative and Antioxidant Defense PCR array (PAHS65) (SABiosciences, Valencia, CA) using real-time PCR. The fold change values of gene expression were determined in BjANN1 cells in comparison to EV-treated controls in three individual experiments using web-based software (http://www.sabiosciences. com/pcrarraydataanalysis.php).