Figure 1: Uptake of globo H by lymphocytes in vitro and in vivo. Human PBMCs were cultured with/without human breast cancer cell, MCF-7. Three days later, PBMCs were harvested from culture medium and the expression of Globo H on PBMC (A) and CD4+ T cells (B) was determined by flow cytometry with mAb VK9. Splenocytes from BALB/c mouse were incubated with synthetic Globo- H-ceramide or ceramide for 3 days, and stained with mAb VK9 to detect the expression of Globo H by flow cytometry (C). (D) Tissue sections of breast cancer with Immunohistochemical staining of VK9 or anti-human CD3 antibody. T: tumor, L: lymphocyte, +: globo H positive, -: globo H negative. (E) The percent of globo H expression in the tumors and infiltrating lymphocytes. T: the percent of globo H positive tumors in 98 clinical breast cancer samples. L: the percent of globo H positive tumors containing globo H positive lymphocytes in 98 samples.