Figure 6: Combination therapy and tNP drug delivery in vitro. Viability of A549Luc cells was assessed by MTT assay after treatment with doxorubicin alone or in combination with IMD-0354 for 48 h (A). Viability of A549Luc cells was measured by the MTT assay after treatment with mitoxantrone alone or in combination with IMD-0354 for 48 h (B). Viability of A549Luc cells was also evaluated by the MTT assay after treatment with cisplatin alone or in combination with IMD-0354 for 48 h (C). Viability of A549Luc cells was determined by the MTT assay after treatment with doxorubicin alone or in combination with IMD-0354 for 48 h under hypoxia (D). Induction of legumain expression under hypoxia was assessed by Western blots of whole cell lysates of A549Luc cells (E). Surface extracellular display of legumain on A549Luc cells was established by FACS under normoxia and hypoxia (F). Relative viability of A549Luc cells assessed by the MTT assay after treatment with tNP-Dox for 48 h and compared to viability after treatment with free Dox (G). Data are shown as mean ± SEM. ***P-value < 0.005.