Figure 2: RT4 cells were treated for 1 hour with the vitamins at their CD90 doses,(VC (8,750μM), VK3 (90μM) and VC:VK3 (520μM:5.2μM)) harvested at one hour intervals for 5 h and assayed for cellular thiol content by monitoring absorbance following reaction with Ellman’s Reagent. Data has been expressed as μM Thiol/mg of protein, calculated on the basis of a GSH standard curve. Values are the mean ± standard error of the mean of three experiments with three readings per experiment. VC:VK3 causes significant depletion of cellular thiols compared to the control (P<<0.0022).