Figure 3: RT-4 cells were treated for 1 hour with the vitamins at their CD90 doses (VC (8,750μM), VK3 (90μM) and VC:VK3 (520μM:5.2μM)) and then harvested at one hour intervals for 5 h. ATP content was assayed using a bioluminescence assay. Data has been expressed as nM ATP per mg of protein and calculated on the basis of an ATP standard curve. Values are the mean ± standard error of the mean of three experiments with three readings per experiment and were compared to the control (P<<0.0022 between VC:VK3 and control from 2-4 hrs).