Cell Line Incubation Time Vitamins Alone Vitamin Combination FIC VC CD50 (μM) VK3 CD50 (μM) VC CD50 (μM) VK3 CD50 (μM) RT4 1 h 4,740 ± 27.2 60.7 ± 4.01 267 ± 4.04 2.68 ± 0.05 0.100 5 days 2,430 ± 28.3 12.8 ± 0.03 110 ± 9.73 1.10 ± 0.10 0.136 T24 1 h 4,970 ± 27.4 73.2 ± 5.91 120 ± 7.0 1.21 ± 0.07 0.093 5 days 1,490 ± 141 13.1 ± 0.01 212 ± 7.6 2.13 ± 0.06 0.158 FIC = CD50A comb/CD50A alone + CD50B comb/CD50B alone, where CD50A alone and CD50B alone are 50% cytopathic doses of each vitamin alone; CD50A comb and CD50B comb are the 50% cytopathic doses of the vitamins administered together. FIC<1.0 is synergistic, FIC>1 is antagonistic and FIC=1 is indifferent

Table 1: Antitumor Activity of Vitamins Against RT-4 Bladder Carcinoma Cells.