Figure 3: Inhibition of TGase 2 promotes cell death in renal cell carcinoma. A. Cytotoxicity of GK921 in human RCC cells exposed to 10-10–10-4 M measured by SRB assay. B. Cells were exposed to 1μM GK921 for 12 h. Annexin V/PI staining was analyzed by flow cytometry. The percentages of gated cells in quadrants corresponding to early and late apoptosis are shown. C. Cells exposed to GK921 for 12 h were subjected to TUNEL assay to detect apoptosis. Bar graph showing the percentage (mean ± SE) of apoptotic cells in at least three randomly selected fields of view (*, p<0.05; **, p<0.01).