Figure 2: Wild type p53 but not mutant p53 (R175H) suppressed endogenous 14-3-3γ protein. HCT116 (p53-/) cells were infected by Adnovirus expressed GFP, wild type p53 and mutant p53 (R175H). After 24 h, 32 h and 48 h, cells were lysed and endogenous 14-3-3γ protein level was analyzed by immunoblotting. Ecotopic expressions of p53 and GFP proteinswere also detected using same membrane. β-actin was used as a loading control. The experiment was repeated three times and a representative data was shown (A). The expression of 14-3-3γ protein seen in the immunoblotting analysis was quantitated on a Strantagene Eagle Eye II, normalized against β-actin and graphed. The graph represents the mean number of the integrated density ±S.D. (n = 3) (B).