Figure 4: p53 suppressed 14-3-3γ by stimulating the process of proteasome-mediated degradation of 14-3-3γ. H358 cells that stably expressed Flag-14-3-3γ protein were infected by Ad-GFP, Ad-p53 (wt) and Ad-p53 (mt). 24 h after infection, the cells were treated with 20 µg/ml cycloheximide plus 10 µm MG-132 for 0 h, 8 h and 24 h and then harvested for detecting Flag-14-3-3γ protein level by immunoblotting. Both wild type and mutant p53 protein were also determined. β-actin was used as a loading control.