Figure 2: Gene expression changes in cancer cell lines after demethylation. The y-axis represents the relative fold difference in mRNA level between 5-dAza treated cells and control and was calculated using the 2–ΔΔCt formula (Paffl, 2001). A) Mean ± SD of 4 experiments for the hNSCLC cells and B) mean ± SD of 3 experiments for the KLN205 cells; asterisk indicates that p < 0.05 vs. control.