Figure 6: Effect of 5-dAza and rotenone on ATP level and ROS production in lung cancer cell line. A) Treatment of cells with rotenone dramatically decreased the ATP level. Application of 5-dAza and rotenone together only slightly restored ATP production. Concentration of ATP in each sample after 24 and 48 hours of 5-dAza and/or rotenone treatment was calculated using standard curve. Bars represent the mean of three experiments ± SD and asterisk indicates that p<0.05 vs. control; B) Cumulative percentage of ROS production after 24 hours cells culture with 5-dAza and/or rotenone. Shift of the curves to the right (▼-5-dAza and ♦ -rotenone) relatively to the control curve indicates increase in ROS production.