Sample ID Treatment Annexin V/PI negative Cells or Live Cells (Mean ± SD)% Annexin V/PI Positive Cells or Apoptotic cells (Mean ±SD)% A 0 mg/mL 96.5 ± 1.1 3.5 ± 1.1 B 5 mg/mL 89.0 ± 0.5 11.0 ± 0.5 C 10 mg/mL 84.5 ± 2.3* 15.5 ± 2.3* D 20 mg/mL 80.0 ± 5.1* 20.0 ± 5.1* E 40 mg/mL 72.7 ± 6.4* 27.3 ± 6.4* F 80 mg/mL 80.8 ± 3.7* 19.2 ± 3.7*

Table 1: Summary data of annexin V assay obtained from the flow cytometry. Human breast adenocarcinoma (MCF-7) cells were cultured in the absence or presence of D-glucose for 2 h as indicated in the Materials and Methods. Values are shown as means ± SDs of 3 replicates per experiment. *P < 0.05 versus compared with control group.