| Target |
Primer sequence forward |
Reverse |
| IDH 1 (Exon 4) |
5’-GCACGGTCTTCAGAGAAGCC-3’ |
5’-CACATTATTGCCAACATGAC-3’ |
| IDH 2 (Exon 4) |
5’-GCCCACACATTTGCACTCTA-3’ |
5’-CAGAGACAAGAGGATGGCTAGG-3’ |
| BRAF (Exon 15) |
5’-TCATAATGCTTGCTCTGATAGGA-3’ |
5’-CTTTCTAGTAACTCAGCAGC-3’ |
| KRAS (Exon 2) |
5’-GTACTGGTGGAGTATTTGATAGTGTATTAA-3’ |
5’-TCAAAGAATGGTCCTGCACC-3’ |
| KRAS (Exon 3) |
5’-CTTTGGAGCAGGAACAATGTCT-3’ |
5’-TACACAAAGAAAGCCCTCCCC-3’ |
| TP53 (Exon 5) |
5’-GTTTCTTTGCTGCCGTCTTC-3’ |
5’-GAGCAATCAGTGAGGAATCAGA-3’ |
| TP53 (Exon 6) |
5’-AGAGACGACAGGGCTGGTT-3’ |
5’-CAAATAAGCAGCAGGAGAAAGC-3’ |
| TP53 (Exon 7) |
5’-AAAAAGGCCTCCCCTGCT-3’ |
5’-TGGAAGAAATCGGTAAGAGGTG-3’ |
| TP53 (Exon 8) |
5’-CAAGGGTGGTTGGGAGTAGA-3’ |
5’-GAGGCAAGGAAAGGTGATAAAA-3’ |
| PTEN (segment 1) |
5’-TTCCATCCTGCAGAAGAAGC-3’ |
5’-GCTGTGGTGGGTTATGGTCT-3’ |
| PTEN (segment 2) |
5’-ACCGCCAAATTTAATTGCAG-3’ |
5’-CGCCACTGAACATTGGAATA-3’ |
| PTEN (segment 3) |
5’-GTGGCACTGTTGTTTCACAAG-3’ |
5’-CTGCACGCTCTATACTGCAAA-3’ |
| PTEN (segment 4) |
5’-ACCAGGACCAGAGGAAACCT-3’ |
5’-AAGGTCCATTTTCAGTTTATTCAAG-3’ |
| EGFR |
5’-TCCCATGATGATCTGTCCCTCACA-3’ |
5’-CAGGAAAATGCTGGCTGACCTAAG-3’ |
| LINE1 |
5’-TGCTTTGAATGCGTCCCAGAG-3’ |
5’-AAAGCCGCTCAACTACATGG-3’ |
| MGMT methylation |
5’-GCGTTTCGACGTTCGTAGGT-3’ |
5’-CACTCTTCCGAAAACGAAACG-3’ |
| Probe (MGMT) |
5’-6FAM-CGCAAACGATACGCACCGCGA-TMR-3’ |
| MGMT expression |
5’-CCGAGGCTATCGAAGAGTTC-3’ |
5’-TCCGAATTTCACAACCTTCA-3’ |
| COL2A1 |
5’-TCTAACAATTATAAACTCCAACCACCAA-3’ |
5’-GGGAAGATGGGATAGAAGGGAATAT-3’ |
| Probe (COL2A1) |
5’-6FAM-CCTTCATTCTAACCCAATACCTATCCCACCTCTAAA-TMR-3’ |
| PTEN expression |
5’-ACCAGGACCAGAGGAAACCT-3’ |
5’-CTGCACGCTCTATACTGCAAA-3’ |
| TBP |
5’-TCGGAGAGTTCTGGGATTGT-3’ |
5’-CACGAAGTGCAATGGTCTTT-3’ |
The table lists all primers used in this study