Target Primer sequence forward   Reverse
IDH 1 (Exon 4) 5’-GCACGGTCTTCAGAGAAGCC-3’ 5’-CACATTATTGCCAACATGAC-3’
IDH 2 (Exon 4) 5’-GCCCACACATTTGCACTCTA-3’ 5’-CAGAGACAAGAGGATGGCTAGG-3’
BRAF (Exon 15) 5’-TCATAATGCTTGCTCTGATAGGA-3’ 5’-CTTTCTAGTAACTCAGCAGC-3’
KRAS (Exon 2) 5’-GTACTGGTGGAGTATTTGATAGTGTATTAA-3’ 5’-TCAAAGAATGGTCCTGCACC-3’
KRAS (Exon 3) 5’-CTTTGGAGCAGGAACAATGTCT-3’ 5’-TACACAAAGAAAGCCCTCCCC-3’
TP53 (Exon 5) 5’-GTTTCTTTGCTGCCGTCTTC-3’ 5’-GAGCAATCAGTGAGGAATCAGA-3’
TP53 (Exon 6) 5’-AGAGACGACAGGGCTGGTT-3’ 5’-CAAATAAGCAGCAGGAGAAAGC-3’
TP53 (Exon 7) 5’-AAAAAGGCCTCCCCTGCT-3’ 5’-TGGAAGAAATCGGTAAGAGGTG-3’
TP53 (Exon 8) 5’-CAAGGGTGGTTGGGAGTAGA-3’ 5’-GAGGCAAGGAAAGGTGATAAAA-3’
PTEN (segment 1) 5’-TTCCATCCTGCAGAAGAAGC-3’ 5’-GCTGTGGTGGGTTATGGTCT-3’
PTEN (segment 2) 5’-ACCGCCAAATTTAATTGCAG-3’ 5’-CGCCACTGAACATTGGAATA-3’
PTEN (segment 3) 5’-GTGGCACTGTTGTTTCACAAG-3’ 5’-CTGCACGCTCTATACTGCAAA-3’
PTEN (segment 4) 5’-ACCAGGACCAGAGGAAACCT-3’ 5’-AAGGTCCATTTTCAGTTTATTCAAG-3’
EGFR 5’-TCCCATGATGATCTGTCCCTCACA-3’ 5’-CAGGAAAATGCTGGCTGACCTAAG-3’
LINE1 5’-TGCTTTGAATGCGTCCCAGAG-3’ 5’-AAAGCCGCTCAACTACATGG-3’
MGMT methylation 5’-GCGTTTCGACGTTCGTAGGT-3’ 5’-CACTCTTCCGAAAACGAAACG-3’
Probe (MGMT) 5’-6FAM-CGCAAACGATACGCACCGCGA-TMR-3’
MGMT expression 5’-CCGAGGCTATCGAAGAGTTC-3’ 5’-TCCGAATTTCACAACCTTCA-3’
COL2A1 5’-TCTAACAATTATAAACTCCAACCACCAA-3’ 5’-GGGAAGATGGGATAGAAGGGAATAT-3’
Probe (COL2A1) 5’-6FAM-CCTTCATTCTAACCCAATACCTATCCCACCTCTAAA-TMR-3’
PTEN expression 5’-ACCAGGACCAGAGGAAACCT-3’ 5’-CTGCACGCTCTATACTGCAAA-3’
TBP 5’-TCGGAGAGTTCTGGGATTGT-3’ 5’-CACGAAGTGCAATGGTCTTT-3’
The table lists all primers used in this study
Table 2: Primers.