| Sample ID |
MGMT |
PTEN |
EGFR [x fold] |
Mutations |
Molecular
sub-classification |
| promoter status |
cDNA expression |
| HROG02 |
tumor |
M |
|
|
3 |
TP53R248Q |
Proneural
TP53 mut; 4q12 (PDGFRA) amplified |
| cell line |
<0.001 |
3.68 |
1 |
| HROG04 |
tumor |
U |
|
|
36 |
PTEN W274L |
Classical
EGFR amplified; 9p21.3 (CDKN2A) deleted |
| cell line |
1.02 |
12.73 |
1 |
| HROG05 |
tumor |
M |
|
|
82 |
K-RasG12D
PTEN P169S/del 212-229 |
Mesenchymal*
PTEN mutated |
| cell line |
<0.001 |
1.01 |
1 |
| HROG06 |
tumor |
U |
|
|
82 |
TP53R273H/R306*
PTEN (+1 at 126) |
Proneural
TP53 mutated; 4q12 (PDGFRA) amplified |
| cell line |
0.07 |
2.31 |
1 |
| HROG07 |
tumor |
U |
|
|
12 |
wt |
Classical
EGFR amplified; 9p21.3 (CDKN2A) deleted |
| cell line |
0.34 |
14.92 |
1 |
| HROG10 |
tumor |
U |
|
|
2 |
wt |
Proneural
4q12 (PDGFRA) amplified |
| cell line |
0.27 |
3.73 |
1 |
| HROG13 |
tumor |
M |
|
|
1 |
wt |
Classical
chr.7 amplified; chr.10 lost; 9p21.3 (CDKN2A) deleted |
| cell line |
<0.001 |
2.88 |
1 |
| HROG15 |
cell line |
M |
<0.001 |
3.70 |
1 |
TP53 R273H
PTEN S170N |
Mesenchymal
PTEN mutated; 17q11.2 (NF) deleted |
| HROG17 |
tumor |
M |
|
|
4 |
PTEN R130* |
Mesenchymal*
PTEN mutated |
| cell line |
<0.001 |
0.55 |
1 |
| HROG24 |
tumor |
U |
|
|
43 |
TP53 R273C
MGMT CN=1
PTEN exon 3 del/spliced |
Proneural
TP53 mutated; 4q12 (PDFRA) amplified |
| cell line |
<0.001 |
2.21 |
1 |
| HROG36 |
tumor |
U |
|
|
1 |
MGMT CN=0
PTEN I5S |
Mesenchymal*
PTEN mutated |
| cell line |
<0.001 |
3.72 |
1 |
| HROG38 |
tumor |
U |
|
|
1 |
PTEN I224M/R234W |
Mesenchymal*
PTEN mutated |
| cell line |
0.23 |
0.02 |
1 |
This table summarizes molecular characteristics of tumors in comparison to the corresponding cell line. The moleclular data was then used for sub-typing according to Verhaak et al. [7]. Listed are the methylation status of the MGMT promoter (M=methylated; U=unmethylated), the relative cDNA expression of the MGMT gene compared to the housekeeping gene TBP, the relative cDNA expression of the PTEN gene compared to the housekeeping gene TBP, the genomic amplification rate of the EGFR compared to the normal diploid status (1=2n) and detected mutations of the genes TP53, PTEN, IDH 1 and 2, KRAS and BRAF (wt=wild type, if no mutations were detected; mutations are indicated by the position with the wt amino acid in front and the amino acid resulting from the mutation behind or * in case of a stop codon; CN=copy number 1, when one copy of the gene was lost and 0 if both copies of the gene were lost; del=deletion of amino acids; spliced=alternatively spliced; +1=insertion of a base leading to a frame shift)