Figure 5: Plasma pharmacokinetic profile of NGD16. (A) The plasma pharmacokinetics was observed following i.v. (5 mg/kg/b.w.) and i.p. (20 mg/ kg b.w.) of NGD16 to male Balb/c mice. Semi-logarithmic concentration-time graph was plotted by taking the quantity of NGD16 in plasma (ng/ml) at each time points taken. The pharmacokinetic parameters and the graphs follow ± SEM of three independent experiments conducted. (B) The quantification of NGD16 was performed using an electrospray ionization (ESI) source in negative mode through SIM on the basis of retention time and the peak areas.