Table 1

Cell lines	NGD16 (µM) IC₅₀ ± s.d.	DIM (µM) IC₅₀ ± s.d.	Staurosporine (µM) IC₅₀ ± s.d.
PC-3	0.8 ± 0.105	32.5 ± 0.239	0.022 ± 0.015
MiaPaca-2	2.8 ± 0.112	15.3 ± 0.153	0.031 ± 0.032
COLO-205	5.3 ± 0.098	43 ± 0.185	0.028 ± 0.054
DU-145	5.9 ± 0.125	76.8 ± 0.188	0.045 ± 0.073
HUVEC	2.5 ± 0.193	25 ± 0.225	0.032 ± 0.135
NIH 3T3	62 ± 0.224	>100	35.5 ± 0.212
IC₅₀ values are indicated as the mean ± s.d. of three independent experiments performed. (s.d.= standard deviation)

Table 1: Effect of NGD16, DIM and Staurosporine (+ve control) on cell viability was determined on a panel of four different cancer cell lines viz: PC-3, MiaPaca-2, COLO- 205, DU-145, HUVECs and NIH 3T3 cells through MTT assay. The N-glycosylated NGD16 found to be 8-10 times more cytotoxic than its parent DIM molecule and its selectivity towards cancer cells. PC-3 and MiaPaca-2 cells were more sensitive to NGD16 with least IC₅₀ values, i.e. 0.8 and 2.8 μM respectively; whereas for HUVECs the IC₅₀ concentration of NGD16 was 2.5 μΜ.