PK Parameters Intravenous (5 mg/kg/b.w.) Intraperitoneal (20 mg/kg/b.w.) Half life (hr) 2.935 ± 0.285 3.32 ± 0.231 Cmax (obs) (ng/mL)   1221.6 ± 0.334 C0 (ng/mL) 3984.4 ± 1.322   Tmax (obs) (hr)   2.0 ± 0.184 AUC0-t (area) (ng*hr/mL) 2731.9 ± 1.212 5108.6 ± 1.261 AUC0-∞ (area) (ng*hr/mL) 2734.6 ± 1.291 5138 ± 2.313 Vd (L/kg) 7.744 ± 0.839 18.68 ± 0.685 CL (L/hr/kg) 1.828 ± 0.162 3.892 ± 0.951

Table 2: The plasma pharmacokinetic profile of NGD16 was assessed following single dose i.v. (5 mg/kg/b.w.) and i.p. (20 mg/kg/b.w.) administration to male Balb/c mice. The plasma drug concentration was quantified with the help of a Triple quadrupole LC-MS system (Agilent Technologies U.S.A) in ESI negative mode through single ion monitoring and the pharmacokinetic parameters were determined using non-compartmental analysis.