Figure 5: Down-regulation of IP-10 in the HM-1/hVASH1 tumors. A: The protein level of IP-10 in the tumors of HM-1/LacZ (N=3) and HM-1/hVASH1 (N=3) mice were compared by Western blot analysis. Means and SDs are shown. B: The expression of IP-10 mRNA in the tumors of HM-1/LacZ (N=3) and HM-1/hVASH1 (N=3) mice were compared by quantitative RT-PCR. Means and SDs are shown. C: Infiltrated T-lymphocytes that had infiltrated into the tumor were immunostained with anti-CD3ε Ab. Scale bar=100 μm. DAPI (blue) was used for nuclear staining. The number of CD3ε-positive T-lymphocytes in the HM-1/LacZ (N=5) and HM-1/hVASH1 (N=5) tumors was compared. Means and SDs are shown.