Figure 2: Depletion of RUVBL2 by siRNA. siRNAs for RUVBL2 (10 nM siRUVBL2-1, -2 and -3) or control siRNAs (10 nM siCONT-H and -M) were transfected into MRC5-SV cells, and the depletion of RUVBL2 was analysed by Western blotting at 3 and 6 days after transfection. Upper panels: typical Western blots for RUVBL2 and β-tubulin (loading control); Lower panels: depletion of RUVBL2 in siRNA-treated cells relative to mock-treated cells. Data points are means of 3 independent experiments with standard deviation.