Figure 1: Effect of apricoxib on IL-27 mediated STAT activation. (A) A549 cells were treated with apricoxib (0.016-2 μM) for 4 hours prior to IL-27 exposure IL-27 (50 ng/mL) for 15 minutes, and the tyrosine phosphorylated forms of STAT1 and STAT3 (P-STAT1; P-STAT3) were detected by Western Blot. The values above the figures represent the relative density of the bands after normalization to GAPDH by densitometry using NIH software, Image J. pSTAT/STAT levels were expressed as fold change with respect to 0 μM apricoxib treated cells. (B) The effect of pretreatment with apricoxib on IL-27 induced STAT activation. A549 cells were pretreated with apricoxib (0.08 μM) up to 48 hours prior to IL-27 (50 ng/mL) exposure for 15 minutes, and expression of STAT1 and STAT3 activation was demonstrated by Western Blot. At this apricoxib dose, pre-treatment for 4 hours was sufficient to augment STAT1 and inhibit STAT3 activation by IL-27. The values above the figures represent relative density of the bands after normalization to GAPDH by densitometry using NIH software, Image J. pSTAT/STAT levels were expressed as fold change with respect to 0 μM apricoxib with IL-27 (0.5 hr) treated cells.