Figure 4: Role of STAT1 in the enhancing effect of apricoxib and IL-27 on the inhibition of EMT. A549 cells were treated with a STAT1 siRNA (40 nM) or a non-targeting control siRNA (40 nM) for 6 hours prior to treatment with IL-27 (50 ng/mL) for 15 minutes and/or apricoxib (0.016-1 μM) for 4 hours. Activated and total STAT1 and STAT3 expression levels (A) and EMT marker expression (B) were demonstrated by Western blot. The values above the figures represent relative density of the bands after normalization to GAPDH.