Figure 2: miR-203 inhibits cell proliferation, survival, migration and invasion in ovarian cancer cells. A. Cell proliferation in SKOV3 and OVCAR3 cells transduced with either lentiviral miR-203 or EGFP controls was examined using MTT assay. Overexpression of miR-203 significantly inhibits cell proliferation in both SKOV3 and OVCAR3 cells compared to that in control cells as analyzed by Student’s T test (*p < 0.05). B. SKOV3 and OVCAR cells transduced with lentiviral miR-203 or EGFP control vectors were seeded into 6-well plates and cultured for 14 d. Cell colonies were counted following Crystal Violet staining. The number of colonies in miR-203-overexpressing cells was significantly reduced compared to that in control cells analyzed by Student’s T test (***p < 0.001). C. Transwell migration assays were performed in miR-203-expressing and control SKOV3 and OVCAR3 cells. Transmigrated cells were stained with Crystal Violet and counted. Overexpression of miR-203 significantly reduced cell migration in both SKOV3 and OVCAR3 cells compared with control cells (***p < 0.001). D. Invasion assays were performed using Matrigel-coated transwell plates for miR-203-expressing and control SKOV3 and OVCAR3 cells. The invasion rate was calculated based on the invasion of miR-203- overexpressing SKOV3 and OVCAR3 cells relative to control cells (**p < 0.01). Data were collected from three separate experiments and analyzed using Student t-tests.