Figure 6: Silencing Snai2 mimics miR-203 function in ovarian cancer cells. A. Cell colony formation assays were performed using SKOV3 and OVCAR3 cells transduced with lentiviral Snai2 and SC shRNA. Cells were cultured for 2 weeks, and then stained with Crystal Violet. *p < 0.05. B. Migrated SKOV3 and OVCAR3 cells transduced with lentiviral Snai2 and SC shRNA were stained using Crystal Violet and counted. **p < 0.01. C. Snai2 knockdown SKOV3, OVCAR3, and control cells were seeded in Matrigel-coated transwells and invaded cells were quantified by measuring the fluorescent intensity following calcein staining. *p < 0.05. D. EMT marker genes in Snai2 knockdown SKOV3 and OVCAR3 cells were determined using Western blot. One representative Western blot was shown from three independent experiments. E. Representative image of immunostaining on Snai2 expression in SKOV3 cells transduced with Snai2 and SC lentiviral shRNAs.