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Figure 4: p53 reporter activation by CCNL2. ras-NIH3T3 cells were co-transfected with firefly reporter plasmids such as pG13-Luc, pGL3-p21-Luc, pGL3-Bax-Luc,pGL3-Bad-Luc, pBV-PUMA Frag2-Luc and pGV-B2 Noxa-Luc and a Renilla reporter plasmid, SV40-Rluc, together with a control empty vector or CCNL2 expression plasmid. Cells were harvested 48 h after transfection and luciferase activities in the cell extracts were measured. Activity of firefly luciferase was normalized by Renilla luciferase activity. The error bars represent SD. *, P < 0.05 and **, P < 0.01 versus vector control, Student’s t test. |