Figure 3: Analysis of human colon cell growth inhibition by L. fermentum NCIMB 5221 using different bacterial cell free extracts. The effect of (a, b, c) the probiotic supernatants (PS) and (d, e, f) the conditioned cell culture medium (CM) of L. fermentum NCIMB 5221 on the viability of colon cancer cells (SW-480) for 12 h, 24 h, and 7 days. **p < 0.05 and ***p < 0.001 compared with L. acidophilus ATCC 314 or L. rhamnosus ATCC 53103. ##p < 0.01 and ###p < 0.001 compared to untreated cells. Data are presented as mean ± SEM (n=4). L. a 314: L. acidophilus ATCC 314; L. rh 53103: L. rhamnosus ATCC 53103; L. f 5221: L. fermentum NCIMB 5221.