| Product |
Strain |
L. fermentum NCIMB 5221 |
| |
Controls |
L. acidophilus ATCC 314 |
| |
|
L. rhamnosus NCIMB 53103 |
| |
|
|
| |
Characterization |
Growth in MRS |
| |
|
Fatty acids levels in bacterial culture |
| |
|
|
| |
Probiotic cell free extracts |
PS: probiotic supernatant (culture based) |
| |
|
Bacterial culture supernatant |
| |
|
CM: conditionned medium (bacterial cell based) |
| |
|
Cell media DMEM treated with bacterial cells |
| |
|
|
| Effect |
Anti-proliferative effect |
Colon cancer cells + PS or CM (12h , 24 h, and 7 dys) |
| |
|
Proliferation and apoptosis |
| |
|
|
| |
Cancer cells vs normal epithelial cells |
CM+ colon cell |
| |
|
CM + non-epithelial colon cells |
| |
|
(1, 2, 3 and 7 d) |
| |
|
|
| Mechanism |
Role of SCFAs produced in CM |
Quantification (lactate, acetate, propionate and butyrate) |
| |
|
Preparation of synthetic SCFA mixtures |
| |
|
|
| |
Testing different mixture of SCFAs |
Decreasing doses of LA, AA, PA and BA and their mixtures |
