Figure 2: Comparative expression analysis of miR-155 and miR-31 in 37 pancreatic cancer patient’s tumor specimens compared to 24 pooled normal samples of FFPE tissue blocks (2A&2D), comparative expression analysis in 24 paired samples of FFPE tissue blocks of tumor and normal tissue samples from the same patient (2B&2E) quantitated individually using qRT-PCR. The Kaplan-Meier curve and Log-ranks tests for miR-155 (2C) and miR-31 (2F) expression and survival of patients are also presented. There was a significant up-regulation of miRNA-155 in all except two tumor samples when compared to normal (2A). Similarly, miR-31 appears to be up-regulated in most of the tumor samples compared to normal samples (2D&2E). The miRNAs expression was normalized using RNU48 miRNA. P values represent comparison between normal and tumor paired samples (2B, 2E) using Wilcoxon matched pairs t-test.