Figure 3: Comparative expression analysis of miR-203 and miR-214 in 37 pancreatic cancer patient’s tumor specimens compared to 24 pooled normal samples of FFPE tissue blocks (3A&3D), comparative expression analysis in 24 paired samples of FFPE tissue blocks of tumor and normal tissue samples from the same patient (3B&3E) quantitated individually using qRT-PCR. The Kaplan-Meier curve and Log-ranks tests for miR-203 (3C) and miR-214 (3F) expression and survival of patients are also presented. There was a significant up-regulation of miRNA-203 and miR-214 in all except few tumor samples when compared to normal (A,B,D and E). Furthermore, Kaplan-Meier curve for miR-214 (3F) demonstrated a significant longer survival of patients with low miR-214 expression. The miRNAs expression was normalized using RNU48 miRNA. P values represent comparison between normal and tumor paired samples (3B,3E) using Wilcoxon matched pairs t-test.