Figure 2: Representative image of in situ hybridization analysis for S-ncmtRNA and AS-ncmtRNA during cervical cancer progression (x200). Normal RAFT organotypic cultures showed expression of both transcripts. Non-tumoral samples showed down-regulation of AS-ncmtRNA. In stages CIN 1, CIN 2, CIN 3 and invasive squamous cancer (SCC), a clear cytoplasmic staining is observed for S-ncmtRNA with down-regulation of AS-ncmtRNA in all these stages. As positive control; parallel sections of each tissue were hybridized with a probe complementary to 18S rRNA.