Figure 3: Standard curves for QPCR efficiency. MCF7 cDNA was reverse transcribed from 1 μg of RNA and serially diluted 10 fold from neat to 1:10, 1:100 and 1:1000. Samples were amplified by QPCR with (A) CTCF, (B) ERα (C) GAPDH and (D) TBP primer pairs using Kapa mastermix. The reaction efficiency, slope and R^2 values for each primer is indicated in the box for each graph. The values were within expected ranges for an accurately optimised QPCR assay.