Figure 5: Effect of Vandetanib on p-Tyrosine and Survivin Expression in RTE Cells. Primary RTE cells were treated with B[ a ]P alone or with five half - log concentrations (0.003, 0.01, 0.03, 0.1 and 0.3 µM ) of Vandetanib and harvested at (A) 14 day (p-Tyrosine) or (B) 28 day (survivin) and processed for Western blot immunoassays. Target proteins were detected by immunoblotting with respective primary antibodies (anti-mouse p-tyrosine or anti-chicken survivin) and alkaline phosphatase-conjugated secondary antibodies (antimouse IgG-AP for p-tyrosine or anti-chicken IgG for survivin). Membrane protein bands were visualized by ECF substrate under Typhoon-fluorescentlaser- imager-9410 and quantitated after normalizing with ß-actin control. The data is expressed as a percent of B[a]P control.