Figure 3: Effect of NAC on ROS production in MDA-MB231 cells treated with leinamycin. The cells were pretreated with 2 mM NAC for 24 h before exposure to various concentrations of leinamycin (0, 3.125, 6.25, 12.5, and 25 ng/mL) for 24 h. The cellular production of ROS was measured using DCFDA and flow cytometry. Figures shown are representative of at least two independent experiments. (B) Graphical representation of data presented in Figure 3A.