Figure 6: Effect of NAC pretreatment on leinamycin-inducible genes in MDA-MB231 cells. The cells were pretreated with 2 mM NAC for 24 h before exposure to leinamycin (0, 12.5, and 25 ng/mL) for 24 h. Semi quantitative RTPCR was used to measure mRNA levels of EGR1, BRCA1, and E2F1 using actin as an internal control.