Figure 3: SDS-PAGE analysis of interferon ß1 purification protocol. SDSPAGE (12 %) analysis of fractions after inclusion bodies washing, solubilization and purification of interferon ß1 protein. M corresponds to Molecular weight marker low range (Biorad). Lane 1 corresponds to soluble fraction after cell disruption. Lane 2 corresponds to soluble fraction after washing of inclusion bodies with 6M urea. Lane 3 corresponds to soluble fraction after wash of inclusion bodies with 2% sulfobetaine 14. Lane 4 corresponds to soluble fraction after solubilization with 2.5% sulfobetaine 14 and a brief incubation at pH 12. Lane 5 corresponds to soluble sample loaded on Sephacryl S200 (first column). Lane 6 corresponds to pooled fractions from Sephacryl S200 column. Lane 7 corresponds to sample loaded on HiTrap CM FF column. Lane 8 corresponds to sample loaded on Sephacryl S200 (third column). Lane 9 corresponds to pooled fractions after the third column (purified protein).