Figure 1: Purification and characterization of Trx/His/Aq-AMP. A. Chromatogram obtained from IMAC purification of Trx/His/Aq-AMP. B. SDS/PAGE 12% of the purified protein. C. Size exclusion chromatography in a Superdex 200 10/300 of the P1 fraction. P1, eluted peak from IMAC purification. MW, molecular weight (kDa), NI, not induced; TI, total induced; BC, before column; AC; after column. Arrow 1 corresponds to the retention volume for Blue Dextran (>700 kDa); arrow 2 corresponds to the retention volume for Ferritin (440 kDa).