Figure 9: Humoral immune response evaluation by ELISA as Th1/Th2 deviation marker. Serum IgG2a and IgG1 antibodies were evaluated 5 weeks after challenge for Th1/Th2 polarized response by Anti-IgG2a/Anti-IgG1 HRP conjugated antibodies. Stimulations were conducted against 2 different peptide pools including P1 and P4 in one pool and P2 and P3 in the other pool. Each column represents mean IgG2a antibody to mean IgG1 antibody level in each group. A. DNADNA immunization. B. DNA-Live immunization.