Figure 1: Flowchart of 2DE proteomic technique. Two-dimensional gel electrophoresis (2DE) is a powerful method for the analysis of complex protein mixtures from biological samples. This technique sorts proteins according to two different properties in discrete steps. First dimension electrophoresis, isoelectric focusing (IEF) will separate complex mixture of proteins according to their isoelectric points (pI). The second dimension step, sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis will further separate the focused proteins based on their molecular weights (M.W.). After image analysis the differentially expressed protein spots will be cut, digested and identified by mass spectrometry.