Figure 3: Western blot analysis of protein phosphorylation by PKA in BAEC. Immunoblot analysis of phospho-PKA substrates in cells cultured in normal (5 mM, NG) or high (22 mM, HG) glucose for 2 weeks. The cells were subjected to static conditions (control) or 3 hours of shear stress (SS). Bar graph shows the densitometric analysis of three independent experiments (means ± SE) normalized to actin controls using UN-SCAN-IT gel 6.1 (*p<0.05). The results showed that shear stress-induced activation of PKA mediated protein phosphorylation was impaired in cells maintained in HG.