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| Figure 2: (a)-(o). In vitro plant regeneration of Carum copticum. (a- b) Germinated seedling (s) on MS and NN media supplemented with 1.0 mg l-1 GA3 and 0.5 mg l-1 GA3 respectively; (c-d) Response of hypocotyl explants on MS and NN media with same level of 1.5 mg l-1 BA for shoot multiplication, (e-f) stage of shoot initiation on MS and NN media respectively, (g) Multiple shoot induction on MS medium with 1.5 mg l-1 BA, (h-i) multiple shoot induction on NN medium with 1.5 mg l-1 BA; (j) Root initiation on MS medium containing 0.25 mg l-1 NAA, (k-l) Roots induction on NN medium supplemented with 0.25 mg l-1 IAA; ( m-o) Stages of acclimatization of plantlets of 2 m old. Bars = 0.57 cm (a-e); 0.5 cm (f-g); 0.71 cm (k-m) and 0.35 cm (n-o). |