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Lawrence J Wangh is currently devoted to the invention and application of new technology in the field of nucleic acid diagnostics. Over the past five years we have discovered and developed a new method of DNA amplification that overcomes the inherent limitations of conventional, symmetric PCR that has been the dominant method of DNA amplification for the last 20 years. In the case of symmetric PCR, each amplicon is generated by the use of two primers of equal concentration and equal Tm (melting temperature) and is therefore double-stranded. Amplification proceeds exponentially, but then slows down and reaches plateau in a stochastic manner. Thus, although such reactions are well known and are often robust, they nevertheless suffer from limitations which are particularly evident in samples containing low numbers of initial targets.
Reproduction Biology and Molecular Cytology
Research Article: J AIDS Clin Res 2015, 6: 441
Research Article: J Mol Biomark Diagn 2014, 5: 175
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