alexa Subrata H Mishra
ISSN: 2379-1764

Advanced Techniques in Biology & Medicine
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Subrata H Mishra

Subrata H Mishra Johns Hopkins University, School of Medicine, USA
Biography

Dr. Mishra received his PhD degree from Georgia State University, Atlanta, GA, USA in 2008. His PhD thesis involved ““Structure and energetics of RNA - protein interactions for HIV RREIIB targeting zinc finger proteins”. He continued in a postdoctoral position at Georgia State University for the departments of Chemistry and Neuroscience until 2010.His PhD thesis involved ““Structure and energetics of RNA - protein interactions for HIV RREIIB targeting zinc finger proteins”. During that period he was also a lecturer in Physical Chemistry for graduate students as well as a MRI course instructor. He moved to Johns Hopkins University, School of Medicine as a postdoctoral Fellow where he works on NMR of large proteins. The system of choice for his current research involves non-ribosomal peptide synthetases (NRPS), large multi-domain multi-modular enzymatic systems, that synthesize a wealth of natural products like antibiotics, anti-cancer agents etc. His current research involves determining the solution structure of a 52 kDa protein which is cyclization domain in the NRPS Yersiniabactin synthetase. He has published in several manuscripts in peer review journals on NMR of large proteins and NMR method development. He also serves on the Review Editorial board of Frontiers in Cellular Biochemistry.

Research Interest

1. Structural Biology of large proteins and protein complexes by Nuclear magnetic resonance (NMR).
2. NMR solution structure determination of proteins, nucleic acids and complexes.
3. Structure-activity relationships of enzymes by biochemical and biophysical methods.
4. NMR Method development.
5. Biochemical and biophysical characterization of Non-ribosomal Peptide syntheses and production of antibiotics.
6. HIV proteins and biologics targeting HIV life cycles.
7. Biomolecular interactions studies by surface plasmon resonance and Isothermal titration calorimetry.

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