Berdja Sihem
Algeria
Research Article
Impact of the Glucolipotoxicity In vivo and Insulinotoxicity Inducing in Fibroblast Aortic of Sand Rat (Psammomys obesus)
Author(s): Smail Leila, Berdja Sihem, Neggazi Samia, Hamlat Nadjiba, Boumaza Saliha, Sahraoui Abdelhamid, Benazzoug Yasmina, Kacimi Ghouti, Haffaf El Mahdi and Aouichat Bouguerra SouhilaSmail Leila, Berdja Sihem, Neggazi Samia, Hamlat Nadjiba, Boumaza Saliha, Sahraoui Abdelhamid, Benazzoug Yasmina, Kacimi Ghouti, Haffaf El Mahdi and Aouichat Bouguerra Souhila
It was established that the sand rat Psammomys obesus is an experimental model of type diabetes 2 and dyslipidemia, in this in vivo study the sand rats were submitted to natural diet associated with fat (10%) and sucrose (20%) enriched diet /rat per day for 6 months.
The initial time and once of the months, plasma parameters were evaluated and at 6th month aortic morphology were examined. To simulate hyperinsulinemic stress, the effects of high dose of insulin 10 UI/ml during 6 h were investigated on subcultured aortic control fibroblasts which were implicated in extracellular matrix remodeling during the atherosclerosis process. In this work report, the proliferation rates, morphological aspects of apoptosis, few markers of oxidative stress (malonaldehyde and nitric oxid) and type I and III collagen contained in extracellular compartment were evaluated. Others animals recei.. View More»
