Journal of Thermodynamics & Catalysis
Open Access
ISSN: 2157-7544
+44 1300 500008
ISSN: 2157-7544
+44 1300 500008
Cheng-Yu Lai
1200 N. DuPont Highway, Dover, DE, 19901
Tanzania
Research Article
Stellate MSN-based Dual-enzyme Nano-Biocatalyst for the Cascade Conversion of Non-Food Feedstocks to Food Products
Author(s): Hao-Hsin Chi, Daniela R Radu, Geraldine Dezayas, Miranda Penney and Cheng-Yu LaiHao-Hsin Chi, Daniela R Radu, Geraldine Dezayas, Miranda Penney and Cheng-Yu Lai
The successful demonstration of a tandem enzymatic catalyst which utilizes stellate macroporous silica nanospheres (Stellate MSN) platform as dual-enzyme host is reported herein. Upon simultaneous loading of beta-glucosidase and glucose isomerase inside their porous structure, Stellate MSNs-featuring a hierarchical pore arrangement and large surface area, show capability to perform a cascade reaction that converts cellobiose, a cellulosic hydrolysis product, into glucose and further to fructose. The silica platform provides a modality for substrate channelling which involves the transfer of the cascade intermediate, glucose, to the next enzyme without first diffusing to the bulk. A key aspect to this proof-of-concept is the two-enzyme system working in an optimized pH domain to fit the modus operandi for both enzymes. The concept could be extrapolated to other enzyme tandems, with pot.. View More»
DOI:
10.4172/2157-7544.1000185