Journal of Computer Science & Systems Biology

Claudio Nicolini

Publications

• Research Article
  SpADS: An R Script for Mass Spectrometry Data Preprocessing before Data Mining
  Author(s): Luca Belmonte, Rosanna Spera and Claudio NicoliniLuca Belmonte, Rosanna Spera and Claudio Nicolini
  
  The recent application of Mass Spectrometry (MS) to Nucleic Acid Programmable Protein Array (NAPPA) technique for proteins identification by non-classical methods leads to the needs of more sophisticated algorithm for peak recognition. NAPPA technique allows for functional proteins to be synthesized in situ directly from printed cDNAs but faces the difficulty generated by the presence of master mix and lysate molecules peaks appearing as background in the overall spectra. A wide range of tools are available to analyze proteins conventional mass spectra corresponding to few molecular species. None of them is optimized for background subtraction. Moreover, peak identification is performed by statistical analysis on characteristics peaks and thus background subtraction can alter outcome by erasing characteristic peaks. A first attempt to overcome the so far d.. Read More»
  DOI: 10.4172/jcsb.1000125

  Abstract PDF