David P De Souza
Australia
Research Article
Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes
Author(s): Liesbet Temmerman, Alysha M De Livera, Jairus B Bowne, John R Sheedy, Damien L Callahan, Amsha Nahid, David P De Souza, Liliane Schoofs, Dedreia L Tull, Malcolm J McConville, Ute Roessner and John M WentworthLiesbet Temmerman, Alysha M De Livera, Jairus B Bowne, John R Sheedy, Damien L Callahan, Amsha Nahid, David P De Souza, Liliane Schoofs, Dedreia L Tull, Malcolm J McConville, Ute Roessner and John M Wentworth
Hyperglycemia causes diabetic nephropathy, a condition for which there are no specific diagnostic markers that predict progression to renal failure. Here we describe a multiplatform metabolomic analysis of urine from individuals with type 2 diabetes, collected before and immediately following experimental hyperglycemia. We used targeted nuclear magnetic resonance spectroscopy (NMR), liquid chromatography - mass spectrometry (LC-MS) and gas chromatography - MS (GC-MS) to identify markers of hyperglycemia. Following optimization of data normalisation and statistical analysis, we identified a reproducible NMR and LC-MS based urine signature of hyperglycemia. Significant increases of alanine, alloisoleucine, isoleucine, leucine, N-isovaleroylglycine, valine, choline, lactate and taurine and decreases of arginine, gamma-aminobutyric acid, hippurate, suberate and N-acetylglutamate were obse.. View More»
