Journal of Cytology & Histology

Eric J Seibel

Publications

• Special Issue Article
  3D Imaging of Fine Needle Aspirates Using Optical Projection Tomographic Microscopy
  Author(s): Kenny F Chou, Qin Miao, Ryan L Coe and Eric J SeibelKenny F Chou, Qin Miao, Ryan L Coe and Eric J Seibel
  
  Conventional Fine Needle Aspiration Biopsy (FNAB) requires minimal tissue sampling and causes minor tissue trauma. For these reasons, it is widely used to diagnose many types of cancers. However, the utility of FNA is limited, due to the lack of sensitivity and loss of structural information. This preliminary study introduces a new type of Three- Dimensional (3D) cytological imaging, Optical Projection Tomographic Microscopy (OPTM) that has the potential to reduce errors in FNAB analysis. We first demonstrate the functionality of OPTM, a microscopic imaging method that produces high resolution 3D images of single cell specimens in absorption mode, to perform multi-cellular imaging of cells stained with hematoxylin. Then, we use OPTM to image entire FNAB-like specimens in their three-dimensional form without experiencing errors from overlapping cells, choice of focal plane, and samplin.. Read More»
  DOI: 10.4172/2157-7099.S2-001

  Abstract PDF