Dentistry

Frans Ramaeckers

Publications

• Research Article
  The Evaluation of Sodium Lauryl Sulphate in Toothpaste on Toxicity on Human Gingiva and Mucosa: A 3D in vitro Model
  Author(s): Bart Vande Vannet, Bart De Wever, Els Adriaens, Frans Ramaeckers and Peter BottenbergBart Vande Vannet, Bart De Wever, Els Adriaens, Frans Ramaeckers and Peter Bottenberg
  
  Five different commercial toothpaste formulations were labeled as A, B, C, D and E and evaluated for toxicity in vitro using reconstituted human oral and gingival mucosa cultures. Toothpastes A, D and E contained sodium lauryl sulphate (SLS) while toothpastes B and C did not. All toothpastes contained fluoride in different quantities. Tissue viability (MTT), tissue morphology (LM and TEM) and release of pro-inflammatory mediator IL-1α were evaluated. For gingival mucosa, topical exposure of toothpastes did not affect MTT values. Examination of oral mucosa however, showed that toothpastes B, E and D induced a significant loss of viability after 1 h (49.2%, 55.5% and 78.4% respectively) (p<0.05). After 3 hours toothpaste B demonstrated a 51.4% loss of viability whereas tissue viability for toothpastes D and E dropped to 11.1% and 4.5% respectiv.. View More»
  DOI: 10.4172/2161-1122.1000325

  Abstract PDF